Fecal screening detects Ebola antibodies in wild great apes, suggests some apes survive exposure

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NEW YORK (September 18, 2014) – A group of international scientists have developed a new method to study Ebola virus in wildlife.

The Wildlife Conservation Society (WCS)-led research, published today in the journal PLOS Neglected Tropical Diseases, describes the use of fecal samples from wild great apes to identify populations likely to have been exposed to the virus. This represents a new tool for performing large, population-scale field assessments that can potentially change the way Ebola virus is studied and improve our understanding of the virus’ distribution in space and time—a matter of great importance to both the human health and conservation communities.

Despite 30 years of Ebola virus research, including on how it is maintained in the wild and how it emerges in wildlife, there is still much to understand. This is due, in part, to the difficulty of capturing and sampling wild animals in Africa’s vast rainforests. Previous Ebola investigations have relied on blood or tissue samples collected from wildlife, but these samples are difficult and expensive to obtain. The methodology published today provides a potential solution; a simple, non-invasive tool for screening large populations of great apes for Ebola virus exposure, providing scientists and conservationists with more information about this mysterious pathogen.

“Ethical issues, as well as the cost and risk involved, make hands-on examination of wild great apes for research problematic,” said WCS field veterinarian Dr. Kenneth Cameron. “This new methodology will make immunologic studies in wild apes safer and much easier, providing us with the ability to answer research questions that simply were beyond reach before.”

The new methodology exploits the fact that, like humans, apes surviving viral infections develop antibodies against them. Typically, those antibodies are measured in the blood. The scientists, however, developed a laboratory technique that can isolate antibodies from ape feces. As part of the study, fecal samples from 80 free-ranging wild gorillas from five different habitats in northern Republic of Congo were collected and tested. Almost half of those were collected within 2-4 years of confirmed Ebola outbreaks in resident apes. Overall, 10% of the samples tested positive, illustrating the success of this test in detecting antibodies against Ebola virus in feces.

Currently, the worst ever human Ebola virus disease (EVD) epidemic is occurring in West Africa. The virus responsible, Zaire ebolavirus, has been associated with human outbreaks and major declines in wild chimpanzee and gorilla populations in Central Africa. Human cases have tended to follow wildlife outbreaks, usually through the consumption of infected wildlife, including great apes and fruit bats.

Avoiding consumption of wildlife found dead can prevent isolated spillover events of the virus from wildlife to humans, and bats sold as food in markets are a clear concern. Once outbreaks take hold in human communities, the public health community is tasked with trying to stop human to human disease transmission through barrier nursing, supportive care, contact tracing, isolation of those who become ill, and education of the public. These are challenging tasks in any situation, let alone in less developed nations where EVD has repeatedly emerged.

WCS veterinarian Alain Ondzie said, “If scientists can better understand patterns of Ebola virus infection in wildlife, the public health sector can be more prepared to prevent human outbreaks.”

How long the great apes’ antibodies persist or whether they confer protection against future infection will require further investigation, but their presence suggests that, as with humans, some apes survive Ebola virus infection. This information will allow researchers to identify regions where Ebola virus has emerged in wildlife and potentially which ape populations are most susceptible.

“It is fantastic that we can get this type of information from simply collecting fecal samples from the forest floor,” said Dr. Patricia Reed, who helped oversee this work as a wildlife veterinarian for the Wildlife Conservation Society (WCS). “From a wildlife conservation perspective, we can use this information to target our limited resources and get more information in-hand more quickly in order to protect great ape populations most at risk for new infections. And the more we learn about the ecology of this pathogen, the more we can proactively assist the public health community.”

With this method, ape feces can be collected over large areas of forest habitat where Ebola is a threat, and levels of antibodies in the sampled animals can be compared over time and between seasons and regions – all without the expense and risks of animal capture and handling. Fecal screening thus opens many new exciting doors to better understand the disease ecology of Ebola and potential management options. For wild great apes this includes longitudinal studies of immune response that can be paired with genetic identification of individuals following outbreaks, identifying unexposed populations for greater anti-poaching protection or future vaccine studies, and monitoring the effectiveness of Ebola vaccines, should such vaccines become a practical reality for free-ranging great apes. This approach could also be adapted to other species that may play roles in Ebola virus transmission, such as small antelope and wild pigs.

The co-authors of the study, “A New Approach for Monitoring Ebolavirus in Wild Great Apes” are:
Patricia E. Reed of WCS (formerly); Sabue Mulangu of National Institutes of Health (NIH); Kenneth N. Cameron of WCS; Alain U. Ondzie of WCS; Damien Joly of WCS (formerly) and Metabiotia, Inc. (Canada); Magdalena Bermejo of the University of Barcelona (Spain); Pierre Rouquet of ECOFAX (Gabon); Giulia Fabozzi of NIH; Michael Bailey of NIH; Zhimin Shen of NIH; Brandon F. Keele of Frederick National Laboratory in Maryland; Beatrice Hahn of the University of Alabama, Birmingham and University of Pennsylvania, Philadelphia; William B. Karesh of WCS (formerly) and Ecohealth Alliance; and Nancy J. Sullivan of NIH.

The article can be found here.

This study was supported by: the United States Fish and Wildlife Service; the Arcus Foundation (www.arcusfoundation.org); the Paul G. Allen Family Foundation; the United States Agency for International Development’s Emerging Pandemic Threats PREDICT program; CSR Tullow Oil Society; the Réseau des Aires Protégées d’Afrique Centrale (RAPAC), the extramural grants (R01 AI50529 and R01 AI58715) and the Intramural Research Program of the U.S. National Institutes of Health; National Institute of Allergy and Infectious Diseases, Vaccine Research Center; among others. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript and the findings do not represent the opinions of the US government.

CONTACT:
Scott Smith – 718-220-3698; ssmith@wcs.org
Stephen Sautner – 718-220-3682; ssautner@wcs.org
Wildlife Conservation Society (WCS)
MISSION: WCS saves wildlife and wild places worldwide through science, conservation action, education, and inspiring people to value nature. VISION: WCS envisions a world where wildlife thrives in healthy lands and seas, valued by societies that embrace and benefit from the diversity and integrity of life on earth. To achieve our mission, WCS, based at the Bronx Zoo, harnesses the power of its Global Conservation Program in more than 60 nations and in all the world’s oceans and its five wildlife parks in New York City, visited by 4 million people annually. WCS combines its expertise in the field, zoos, and aquarium to achieve its conservation mission. Visit: www.wcs.org. Follow: @thewcs.